G O ALPHA; MS X 100UG

Code: MAB3073 D2-231

Application

Research CategorySignaling

Anti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB.

Research Sub CategoryGP...


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Your Price
€500.00 EACH
€615.00 inc. VAT

Application

Research CategorySignaling

Anti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB.

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Western blot 1:3000.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Bovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide as a preservative.

Specificity

Go-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have >95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 µl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone2A, monoclonal
Gene Informationhuman ... GNAO1(2775)
isotypeIgG1
manufacturer/tradenameChemicon®
NCBI accession no.NM_020988.1, NM_138736.1
Quality Level100
shipped inwet ice
species reactivityguinea pig, human, bovine, rat, mouse
technique(s)western blot: suitable
UniProt accession no.P29777
This product has met the following criteria to qualify for the following awards:



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